《植物生理学报》 2011, 47(6): 607-612

外源24-表油菜素内酯对盐胁迫下茄子种子萌发和幼苗生理特性的影响

吴雪霞, 查丁石*, 朱宗文, 李贤

上海市农业科学院园艺研究所, 上海市设施园艺技术重点实验室, 上海201403

通信作者：查丁石；E-mail: dingshizha@yahoo.com；Tel: 021-62207992

摘　要：

研究了150 mmol•L-1 NaCl胁迫下, 外源24-表油菜素内酯(EBR)对茄子种子萌发、幼苗生长和生理特性的影响。结果 表明, 0.05 mg•L-1外源EBR显著缓解150 mmol•L-1 NaCl的胁迫伤害, 使茄子种子发芽率提高了8.23%, 发芽势提高15.91%, 发 芽指数提高了17.23%, 活力指数提高了44.29%; 幼苗株高、根长和植株鲜重分别提高了56.67%、23.83%和56.68%; 抗氧化 酶(SOD、POD、CAT和APX)活性分别增加了13.75%、24.00%、28.64%和21.46%, 脯氨酸和可溶性糖含量分别提高30.96%和23.66%; MDA含量、O·ˉ产生速率分别降低了29.58%和14.80%。表明0.05 mg•L-1外源EBR能显著促进盐胁迫下茄 子种子萌发和幼苗生长, 明显缓解叶片氧化损伤, 增强茄子的耐盐能力。

关键词：24-表油菜素内酯; NaCl胁迫; 茄子; 种子萌发; 氧化损伤

收稿：2011-03-18   修定：2011-03-18

资助：上海市科技兴农重点公关项目[沪农科攻字(2009)第2-1号]、上海市自然科学基金(ZR1426800)和现代农业产业技术体系建设专向资金

Effects of Exogenous 24-Epibrassinolide on Seed Germination, Physiological Characteristics of Eggplant Seedlings under NaCl Stress

WU Xue-Xia, ZHA Ding-Shi*, ZHU Zong-Wen, LI Xian

Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

Corresponding author: ZHA Ding-Shi; E-mail: dingshizha@yahoo.com; Tel: 021-62207992

Abstract:

The objective of this study was to investigate the effects of exogenous 24-epibrassinolide (EBR) on seed germination, seedling growth, and physiological characteristics in eggplant (Solanum melongena L.) seedlings under 150 mmol·L^-1 NaCl stress. The results showed that 0.05 mg·L^-1 EBR significantly alleviated the damage caused by 150 mmol·L^-1 NaCl. Specifically, seed germination rate, germination potential, germination index and vigor index were increased by 8.23%, 15.91%, 17.23%, and 44.29%, respectively; plant height, root length and fresh weight were increased by 56.67%, 23.83% and 56.68%, respectively; the activities of SOD, POD, CAT and APX were increased by 13.75%, 24.00%, 28.64% and 21.46%, respectively; and contents of proline and soluble sugar were increased by 30.96% and 23.66%, respectively; while contents of MDA and O₂^·-producing rate were decreased by 29.58% and 14.80%, respectively. Results suggested that exogenous 0.05 mg·L^-1 EBR significantly stimulated seed germination and seedling growth, alleviated oxidative damage, and elevated the salt tolerance of plant.

Key words: 24-epibrassinolide; NaCl stress; eggplant; seed germination; oxidative damage